A simple and reliable method for correlative light and electron microscopic studies.

A simple and reliable method for correlative light and electron microscopic studies.

We describe in detail a simple method for flat-embedding that can be subsequently used in correlative light and electron microscopic studies. The method can be applied to any material suitable for electron microscopy and is especially useful for study of the synaptology and ultrastructural characteristics of immunocytochemically or morphologically identified neurons or their processes. We prese...

Starting Dose Calculation for Medicinal Plants in Animal Studies; Recommendation of a Simple and Reliable Method

Background and purpose: Research studies indicate that almost 80% of the present day drugs are derived directly or indirectly from medicinal plants. The estimation of a safe starting dose is a concern when a new substance is to be investigated including medicinal plants, in clinical and laboratory studies. This study was intended to explore a simple and reliable method of calcu...

Correlative light and electron microscopic exploration of endocytic organelles

Explorations of endocytic pathways teach us about subcellular routes travelled by pathogenic agents, such as viruses and toxins, and used for targeting of drugs into the interior of cells. In this compendium we show a correlative microscopic approach that uses the photooxidation capacity of fluorescent dyes to oxidize the substrate diaminobenzidine which after osmification is visible in the ele...

light and electron microscopic studies of phyarynx and osophagus of barbussharpyi fish

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Light and electron microscopic studies on chicken intestinal globule leucocytes.